'Water Microbiology\r'

'Alyse Rose Microbiology Lab Bacterial Unknown process 25, 2013 Bacterial Unknown Report Each disciple was given an transcendental bacteria to figure out. I was given the unknown bacteria S38. Everybody is supposed to do all sorts of test to identify the bacteria. The first occasion I did was smear my bacteria on a liquid intermediate. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to constant of gravitation foul it.This is utilise to differentiate the bacteria. The different patch reagents are: crystal violet, grams iodine, acetone-alcohol, and grams safranin. Under the microscope it was a pick apart color, which means Gram Negative. Also, the shape was a rod. 2. KLIGER’S IRON AGAR (KIA) The next step I did was the KIA dip. This is for the Negative bacteria to see if they are in the Enteriobacteriaceae group. There are many different results than croupe occur, like: 1.Yellow slant/yell ow belatedly- (A/A) glucose & lactose are fermented; if the simplyt has bubbles in it or is displaced, the thermionic valve is gas absolute. 2. Red slant/yellow deep- (K/A) glucose but not lactose is fermented 3. Red slant/ forbidding deep- (K/A) glucose but not lactose is fermented and H2S2 is produced. The deep is battery-acid (yellow) 4. Red slant/ red deep- (K/NC) neither moolah is fermented. Peptone is degraded aerobically My result was a red slant and black deep. When is H2S2 is produced.\r\nRead Chapter 8 Microbial GeneticsThe deep is acid (yellow). Glucose was fermented and lactose wasn’t. 3. UREASE HYDROLYSIS TEST This test is used to see if urea is used. Proteus can be determined because there fast urease activity. Proteus is the energy to utilize lactose. My test resulted in a positive result. It was a pink color. In my end result, my unknown bacteria is Proteus Vulgaris. Gram Stain| commanding Gram Stain| Negative gram stain| KIARed slant/black deep| Ur ease Test| Urease confident(p)| Urease Negative| Proteus Vulgaris|\r\n'